Recombinant Human CXCL10/IP-10/CRG-2

Molecule:

CXCL10/IP-10

Synonyms:

Interferon-inducible Protein 10, C7, crg-2, gIP-10, IFI10, INP10, SCYB10

Species:

Human

Cat. No.:

CRI300A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$190.00 BUY

Description:

Recombinant Human CXCL10/IP-10 is a single non-glycosylated polypeptide chain containing 77 amino acids.

Background: Gamma -Interferon Inducible Protein 10 (IP-10)/CXCL10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that IP-10 mRNA is also induced by LPS, IL-1beta, TNF-alpha, IL-12 and viruses. Additional cell types that have been shown to express IP-10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. IP-10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human IP-10.

Entrez Symbol:

CXCL10

Data PDF:

CRI300

Source:

E. coli

Molecular Weight:

~8.6 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyse.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuIP-10/CXCL10 as determined by LAL method.

Amino Acid Sequence:

VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C.