Recombinant Human CXCL11/I-TAC

Molecule:

CXCL11

Synonyms:

H174, I-TAC, IP-9, IP9, SCYB11, SCYB9B, b-R1, small inducible cytokine B11, small inducible cytokine subfamily B (Cys-X-Cys), member 11, small inducible cytokine subfamily B (Cys-X-Cys), member 9B

Species:

Human

Cat. No.:

CRI600C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Human I-TAC is a single non-glycosylated polypeptide chain containing 73 amino acids.

Background: I-TAC/CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-γ and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T-cells, neutrophils or monocytes.

Entrez Symbol:

CXCL11

Data PDF:

CRI600

Source:

E. coli

Molecular Weight:

~8.3 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4 + 100 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyse.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human IL-2 activated human T-lymphocytes is in a concentration range of 0.1-10 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuI-TAC/CXCL11 as determined by LAL method.

Amino Acid Sequence:

FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQ ARLIIKKVER KNF

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C to -80°C. Avoid repeated freeze/thaw cycles