Recombinant Human CXCL2/GRO beta

Molecule:

CXCL2

Synonyms:

CINC-2a, CINC-3, GRO2, GROb, HSF, MGSA beta, MIP-2, MIP2-alpha, SCYB2

Species:

Human

Cat. No.:

CRG501C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Human CXCL2/GRO-beta is a single non-glycosylated polypeptide chain containing 73 amino acids.

Background: The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Data PDF:

CRG501

Source:

E. coli

Molecular Weight:

~7.9 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4 + 50 mM NaCl.

Purity:

>97% by HPLC and SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.

Endotoxin Level:

Less than 1EU/μg as determined by LAL method.

Amino Acid Sequence:

APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.