Recombinant Human CXCL5/ENA-78

Molecule:

CXCL5

Synonyms:

Epithelial Neutrophil-Activating Protein 78, ENA-78, SCYB5, Neutrophil Activating Peptide ENA-78, Small-inducible cytokine B5

Species:

Human

Cat. No.:

CRE300B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$235.00 BUY

Description:

Recombinant Human CXCL5/ENA78 is a single non-glycosylated polypeptide chain containing 74 amino acids.

Background: Human ENA-78/CXCL5(5-78a.a.) Epithelial cell-derived neutrophil-activating peptide 78 (ENA-78) is a member of the CXC subfamily of chemokines that has the Glu-Leu-Arg (ELR) motif preceding the CXC motif. Similar to other ELR containing CXC chemokines, ENA-78 is a potent neutrophil chemoattractant and activator. Proteolysis of ENA-78 with cathepsin G and chymotrypsin have yielded N-terminally truncated variants with increased biological activities. ENA-70 and ENA-74 represent truncated recombinant ENA-78 variants missing 8 and 4 aa residues, respectively, from the N-terminus. Recombinant ENA-70 and ENA-74 have been shown to have increased potency in neutrophil chemotaxis and myeloperoxidase and elastase release assays.

Entrez Symbol:

CXCL5

Uniprot Name:

C-X-C motif chemokine 5

Uniprot ID:

P42830

Data PDF:

CRE300

Source:

E. coli

Molecular Weight:

~8.1 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 5.0-10 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuENA-78/CXCL5 as determined by LAL method.

Amino Acid Sequence:

AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C.