Recombinant Human IL-8/CXCL8 (77 aa)

Molecule:

IL-8/CXCL8

Synonyms:

NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1; IL8, interleukin-8, emoctakin, , C-X-C motif chemokine 8, T cell chemotactic factor, T-cell chemotactic factor, neutrophil-activating peptide 1, chemokine (C-X-C motif) ligand 8

Species:

Human

Cat. No.:

CRI110B pdf (datasheet)

Quantity/Size:

25 μg

Price:

$235.00 BUY

Description:

Recombinant Human IL-8 is a single, non-glycosylated polypeptide chain containing 77 amino acids.

Background: Interleukin-8 (IL-8)/CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocytederived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

Entrez Symbol:

IL8

Uniprot Name:

Interleukin-8

Uniprot ID:

P10145

Data PDF:

CRI110

Source:

E. coli

Molecular Weight:

~8.9 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Purity:

>96% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

Endotoxin Level:

Less than 1EU/μg of rHuIL-8/CXCL8(77a.a.)as determined by LAL method.

Amino Acid Sequence:

AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon re-constitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.