Recombinant Human D4 GDI/RhoGDI-2, GST-tagged

Molecule:

D4GDI

Synonyms:

D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2, Rho GDI 2, rho-GDI beta, D4-GDI

Species:

Human

Cat. No.:

CRD112C pdf (datasheet)

Quantity/Size:

10 μg

Price:

$695.00 BUY

Description:

D4-GDI belongs to the family of Rho-GDIs or GDP-dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI-1, D4 GDI/RhoGDI-2 and RhoGDI-3. It inhibits the release of GDP from the Rho proteins, essential for GTP-loading and activation of GTPase activity. D4-GDI is located in the cytoplasm and is involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas-mediated apoptosis.

D4-GDI is expressed only in hematopoietic tissues, mainly in B and T cells, unlike RhoGDI which is expressed ubiquitously. D4-GDI plays a role in the regulation of lymphocyte activation, survival, and responsiveness. Expression of D4-GDI is also observed in cells of nonhematopoietic neoplasms, including ovarian and bladder cancer cells, suggestive of D4-GDI’s role in progression of human tumors.

Recombinant Human D4 GDI/RhoGDI-2 is comprised of the full length protein with a GST tag.

Entrez Symbol:

ARHGDIB

Uniprot Name:

Rho GDP-dissociation inhibitor 2

Uniprot ID:

P52566

Data PDF:

CRD112

Source:

E. coli

Molecular Weight:

54 kDa (D4 GDI predicted MW ~23 kDa + GST tag)

Tag:

GST tag

Physical State:

Liquid

Formulation:

Liquid in a sterile filtered solution in 50 mM Tris-HCI, pH 7.5, + 10 mM L-Glutathione (reduced).

Purity:

>90% by SDS-PAGE analysis.

Storage/Stability:

Store product at -80°C. Stable for 1 year. For multiple use, aliquot and freeze at -80°C. Avoid repeated freeze-thaw cycles.

Application notes:

ELISA, Western Blot, Functional Assays