Recombinant Human EPOα

Molecule:

EPO

Synonyms:

EPO; EP; MVCD2; erythropoietin

Species:

Human

Cat. No.:

CRE600A pdf (datasheet)

Quantity/Size:

500 U

Price:

$195.00 BUY

Description:

Recombinant Mature human EPO contains 166 amino acid residues.

Background: Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients. The cDNAs for EPO have been cloned from human, mouse, dog, etc. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level. Human EPO cDNA encodes a 193 amino acid residue precursor protein that is processed to yield a 165 amino acid residue mature protein. EPO contains one O-linked and three N-linked glycosylation sites. Glycosylation of EPO is required for EPO biological activities in vivo. EPO exhibits structural as well as amino sequence identity to the amino terminal 153 amino acid region of thrombopoietin.

Entrez Symbol:

EPO

Uniprot Name:

Erythropoietin

Uniprot ID:

P01588

Data PDF:

CRE600

Source:

CHO cells

Molecular Weight:

Predicted molecular mass of ~21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 36-40 kDa in SDS-PAGE.

Formulation:

Sterile filtered liquid in sodium citrate buffer (1 liter of ddH2O containing 5.9 g of sodium citrate, 5.8 g of sodium chloride and 0.06 g of citric acid).

Purity:

>98% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and was found to be no less than 1.5 x 10E5 IU/mg.

Specific Activity:

>1.5 x 10E5 IU/mg

Endotoxin Level:

<0.01 EU/μg of recombinant human EPOα as determined by LAL method.

Amino Acid Sequence:

APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

Storage/Stability:

Store at 2 to 8°C under sterile conditions after opening. Do not freeze.