Recombinant Human KGF-2/FGF-10

Molecule:

FGF10

Synonyms:

KGF-2, FGF-10

Species:

Human

Cat. No.:

CRK001C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$4,680.00 / Special Price: $1,890.00 BUY

Description:

Recombinant Human FGF-10 is composed of 169 amino acid residues (aa 40 - 208).

Background: Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant human FGF-10 contains 208 amino acids and it shares 92% and 95% amino acid sequence identity with mouse and rat FGF-10. Defects in FGF-10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands and lacrimo-auriculo-dento-digitial syndrome.

Entrez Symbol:

FGF10

Uniprot Name:

Fibroblast growth factor 10

Uniprot ID:

O15520

Data PDF:

CRK001

Source:

E. coli

Molecular Weight:

~19.1 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

Endotoxin Level:

<1 EU/µg of recombinant human FGF-10 as determined by LAL method.

Amino Acid Sequence:

LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C. Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.