Recombinant Human FGF-22

Molecule:

FGF22

Species:

Human

Cat. No.:

CRF037D pdf (datasheet)

Quantity/Size:

100 μg

Price:

$640.00 BUY

Description:

Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Human FGF-22 is a member of the FGF-7 subfamily and is synthesized by multiple cell lines including neurons, keratinocytes and skeletal muscle myotubes. Human FGF-22 shares 86% homology with mouse FGF-22. Recombinant human FGF-22 is a non-glycosylated protein, containing149 amino acids.

Data PDF:

CRF037

Source:

E. coli

Formulation:

Recombinant human FGF-22 is lyophilized with no additives.

Purity:

≥ 97% as determined by HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm.

Biological Activity:

The activity, as determined by the dose-dependent proliferation of 4MBr-5 cells, is typically 50-300 ng/ml.

Endotoxin Level:

Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.

Amino Acid Sequence:

MTPSASRGPR SYPHLEGDVR WRRLFSSTHF FLRVDPGGRV QGTRWRHGQD SILEIRSVHV GVVVIKAVSS GFYVAMNRRG RLYGSRLYTV DCRFRERIEE NGHNTYASQR WRRRGQPMFL ALDRRGGPRP GGRTRRYHLS AHFLPVLVS

Reconstitution:

It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Storage/Stability:

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C.