Recombinant Human FGF1/FGF-acidic (aa 141)

Molecule:

FGF1/FGF-acidic

Synonyms:

AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, HBGF-1, HBGF1

Species:

Human

Cat. No.:

CRF000C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,350.00 / Special Price: $950.00 BUY

Description:

Recombinant Human FGF acidic is a single, non-glycosylated polypeptide chain containing 141 amino acids.

Background: Fibroblast Growth Factor acidic (FGF acidic), also known as FGF-1 and endothelial cell growth factor, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named “acidic fibroblast growth factor" (FGF1) and “basic fibroblast growth factor” (FGF2). Human FGF-acidic shares 54% amino acid sequence identity with FGF-basic. In mammalian FGF receptor family has 4 members, FGFR1, FGFR1, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types “b”, “c”. aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparin sulfate proteoglycan. FGF-acidic plays an important role in the regulation of cell survival, cell division, antiogenesis, cell differentiation and cell migration. FGF-acidic is also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion.

Entrez Symbol:

FGF1

Uniprot Name:

Heparin-binding growth factor 1

Uniprot ID:

P05230

Data PDF:

CRF000

Source:

E. coli

Molecular Weight:

~16.0 kDa

Formulation:

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Purity:

>95% by SDS-PAGE and HPLC analyses.

Biological Activity:

The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg in the presence of 10 μg/ml of heparin.

Endotoxin Level:

<1 EU/μg as determined by LAL method.

Amino Acid Sequence:

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Storage/Stability:

The lyophilized protein is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. Reconstituted protein is stable for 1 week at 2-8°C.