Recombinant Human FGF2/FGF-basic

Molecule:

FGF2/FGF-basic

Synonyms:

BFGF, FGFB, HBGF-2

Species:

Human

Cat. No.:

CRF001C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,080.00 / Special Price: $725.00 BUY

Description:

Recombinant Human Fibroblast Growth Factor Basic is a single, non-glycosylated polypeptide chain containing 147 amino acids. An alternate form, containing 154 amino acids, also exists, but there is no detectable difference in biological activity between the 154aa and 147aa forms.

Background: Human Fibroblast Growth Factor basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages and has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, tumor growth and angiogenesis. Multiple forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the FGF2 gene.

Entrez Symbol:

FGF2

Uniprot Name:

Heparin-binding growth factor 2

Uniprot ID:

P09038

Data PDF:

CRF001

Source:

E. coli

Molecular Weight:

16.5 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered solution in 10 mM sodium phosphate, pH 8.0.

Purity:

>96% by HPLC and SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, typically less than 5 ng/ml.

Specific Activity:

> 1 x 10e5 units/mg

Endotoxin Level:

≤1 U/µg of protein by kinetic LAL analysis (50% confidence range).

Amino Acid Sequence:

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.