Recombinant Human FGF21

Molecule:

FGF21

Synonyms:

Fibroblast growth factor 21, FGF-21

Species:

Human

Cat. No.:

CRF159B pdf (datasheet)

Quantity/Size:

25 μg

Price:

$235.00 BUY

Description:

Recombinant Human FGF21 is a single non-glycosylated polypeptide chain containing 181 amino acids.

Background: Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene and is specifically induced by HMGCS2 activity. All FGF family members are heparin binding growth factors with a core 120 amino acid (aa) FGF domain. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB.  FGF-21 contains a 28 aa signal sequence and a 181 aa mature region but shows limited binding to heparin.

Entrez Symbol:

FGF21

Data PDF:

CRF159

Source:

E. coli

Molecular Weight:

~19.5 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2

Purity:

>95% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml.

Specific Activity:

> 2.0 × 103 IU/mg in the presence of 5 µg/ml of recombinant mouse Klotho-β and 10 μg/ml of heparin.

Endotoxin Level:

< 1 EU/µg as determined by LAL method.

Amino Acid Sequence:

HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Storage/Stability:

Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C.