Recombinant Human FLT3 Ligand

Molecule:

FLT3L

Synonyms:

fms-related tyrosine kinase 3 ligand, FLT3L, SL Cytokine

Species:

Human

Cat. No.:

CRF110C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,510.00 / Special Price: $2,170.00 BUY

Description:

Recombinant Human FLT3 Ligand is a single non-glycosylated polypeptide chain containing 155 amino acids.

Background: Flt-3 ligand (Flt-3L) is a hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3. Flt-3L is structurally related to MCSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human Flt-3L isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced Flt-3L mRNA, encoding a soluble form of the human FLT-3L, has also been identified. Flt-3L is widely expressed in various human and mouse tissues. Flt-3L has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors. Furthermore, Flt-3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.

Entrez Symbol:

FLT3LG

Uniprot Name:

SL cytokine

Uniprot ID:

P49771

Data PDF:

CRF110

Source:

E. coli

Molecular Weight:

17.6 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

>95% by HPLC and SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by proliferation of OCI-AML5 cells, typically <10 ng/ml.

Specific Activity:

≥1 x 10e5 units/mg

Endotoxin Level:

≤1 U/µg of protein by kinetic LAL analysis (50% confidence range).

Amino Acid Sequence:

MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.