Recombinant Human G-CSF/CSF3

Molecule:

CSF3

Synonyms:

CSF3OS, GCSF, granulocyte colony-stimulating factor, filgrastim, lenograstim, pluripoietin

Species:

Human

Cat. No.:

CRG300B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. Human and mouse G-CSF are cross-reactive.

Recombinant Human Granulocyte-Colony Stimulating Factor is a single non-glycosylated polypeptide chain containing 175 amino acids.

Entrez Symbol:

CSF3

Uniprot Name:

Granulocyte colony-stimulating factor

Uniprot ID:

P09919

Data PDF:

CRG300

Source:

E. coli

Molecular Weight:

~18.8 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic acid (TFA).

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by dose-dependent proliferation by NFS-60 cells, is ≤50 pg/ml.

Specific Activity:

>2 x 107 IU/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis.

Amino Acid Sequence:

MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C.