Recombinant Human GDNF

Molecule:

GDNF

Synonyms:

GDNF, ATF1, ATF2, HSCR3, HFB1-GDNF

Species:

Human

Cat. No.:

CRG400A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$135.00 BUY

Description:

Recombinant Human GDNF is a non-glycosylated homodimer, containing two 135 amino acid chains.
Background: Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a neurotrophic factor that is closely related to other neurotrophic factors, such as Neurturin, Persephin, and Artemin, by a common structural feature called the cysteine-knot. GDNF signals through a multicomponent system of receptors that includes RET and GFRα1-4, to promote dopamine uptake, survival and differentiation of neurons.

Entrez Symbol:

GDNF

Uniprot Name:

Glial cell line-derived neurotrophic factor

Data PDF:

CRG400

Genbank Accession No:

P39905

Source:

E. coli

Molecular Weight:

30.4 kDa (monomer 15.2 kDa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution in 10 mM Sodium Citrate + 100 mM NaCl, pH 4.0

Purity:

>95% as determined by SDS-PAGE

Biological Activity:

The biological activity as determined by the dose-dependent proliferation of C6 cells is typically 0.5-5 μg/mL.

Specific Activity:

2 x 10E3 units/mg

Endotoxin Level:

<1 EU/µg of protein (50% confidence range).

Amino Acid Sequence:

MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized protein is stable at room temperature for shipping purposes. Store desiccated at -20°C on receipt. After reconstitution, stable for ≤1 month at 2-8°C. For long term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.