Recombinant Human GM-CSF/CSF2

Molecule:

CSF2

Synonyms:

GMCSF, CSF, molgramostin, sargramostim, colony-stimulating factor, granulocyte-macrophage colony stimulating factor

Species:

Human

Cat. No.:

CRG100B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$235.00 BUY

Description:

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. GM-CSF is a growth factor for granulocyte-macrophage, erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells.

Recombinant Human GM-CSF is a single non-glycosylated polypeptide chain containing 128 amino acids.

Entrez Symbol:

CSF2

Uniprot Name:

Granulocyte-macrophage colony-stimulating factor

Uniprot ID:

P04141

Data PDF:

CRG100

Source:

E. coli

Molecular Weight:

~14.6 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered solution in 10 mM sodium phosphate, pH 7.5.

Purity:

>95% by SDS-PAGE

Biological Activity:

The protein has full biological activity when compared to the standard. The ED50, as determined by dose-dependent proliferation assay using human TF-1 cells is 0.5 - 0.1 ng/ml.

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis (50% confidence range).

Amino Acid Sequence:

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.