Recombinant Human IFN-alpha 2a

Molecule:

IFN-alpha

Synonyms:

IFNA, INFA2, interferon alpha-2, leIF A, IFN-alpha-2, interferon alpha A, interferon alpha-A, alpha-2a interferon, interferon alpha 2b

Species:

Human

Cat. No.:

CRI003C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

This Product is Discontinued

Description:

Recombinant Human IFN-alpha 2a is a single non-glycosylated polypeptide chain containing 165 amino acids. Background: IFN-alphas are proteins secreted by leukocytes. They are mainly involved in innate immune response against viral infection. The IFN-alpha family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Entrez Symbol:

IFNA2

Uniprot Name:

Interferon alpha-2

Uniprot ID:

P01563

Data PDF:

CRI003

Source:

E. coli

Molecular Weight:

~19.2 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 x 108 IU/mg.

Endotoxin Level:

<1 EU/μg as determined by LAL method.

Amino Acid Sequence:

CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C. Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.