Recombinant Human IFN-alpha 2b (E. coli)

Molecule:

IFN-alpha

Synonyms:

Interferon alpha 2b, IFNA, INFA2

Species:

Human

Cat. No.:

CRI004C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$900.00 / Special Price: $375.00 BUY

Description:

Recombinant Human IFN-alpha 2b is a single non-glycosylated polypeptide chain containing 166 amino acids. Background: IFN-alphas are proteins secreted by leukocytes. They are mainly involved in innate immune response against viral infection. The IFN-alpha family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Entrez Symbol:

IFNA8

Uniprot Name:

Interferon alpha-8

Uniprot ID:

P32881

Data PDF:

CRI004

Source:

E. coli

Molecular Weight:

~19.4 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.

Purity:

>96% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The specific activity as determined by an anti-viral assay is ≥1.0 x 10e8 IU/mg.

Endotoxin Level:

<0.1 ng/µg of Recombinant Human IFN-alpha 2b as determined by LAL method

Amino Acid Sequence:

MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

After receipt, store at ≤20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.