Recombinant Human IFN-gamma

Molecule:

IFN-gamma

Synonyms:

IFG, IFI, interferon gamma, IFN-gamma, immune interferon

Species:

Human

Cat. No.:

CRI000C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,000.00 / Special Price: $690.00 BUY

Description:

Interferon-gamma (IFN-gamma), also known as Type II interferon or immune interferon, is a cytokine produced primarily by lymphocytes and natural killer cells. The protein shares no significant homology with IFN-beta or the various IFN-alpha family proteins. Mature IFN-gamma exists as noncovalently-linked homodimers. Human IFN-gamma is highly species specific and is biologically active only in human and primate cells. IFN-gamma was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-gamma also augments TH1 cell expansion and may be required for TH1 cell differentiation.

Recombinant Human IFN-gamma is a single non-glycosylated polypeptide chain containing 144 amino acids.

Entrez Symbol:

IFNG

Uniprot Name:

Interferon gamma

Uniprot ID:

P01579

Data PDF:

CRI000

Source:

E. coli

Molecular Weight:

~16.9 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered solution in 5 mM sodium phosphate + 50 mM sodium chloride, pH 7.2.

Purity:

>95% by HPLC and SDS-PAGE

Biological Activity:

Activity is measured by antiviral assay using EMC-infected A549 cells.

Endotoxin Level:

≤0.5 EU/µg of protein by kinetic LAL analysis (50% confidence range).

Amino Acid Sequence:

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFK N FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVM AE LSPAAKTGKR KRSQMLFQGR RASQ

Reconstitution:

Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Upon reconstitution, a small amount of precipitate may remain. 10% overfill has been added to compensate.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.