Recombinant Human IGF-1

Molecule:

IGF1

Synonyms:

Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF

Species:

Human

Cat. No.:

CRI500A pdf (datasheet)

Quantity/Size:

20 μg

Price:

$135.00 BUY

Description:

Recombinant Human IGF-1 is a single non-glycosylated polypeptide chain containing 70 amino acids.

Background: The insulin-like growth factors (IGFs) belong to the insulin gene family, and are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Mature human IGF-1 shares 94 % and 96 % a.a. sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.

Entrez Symbol:

IGF1

Uniprot Name:

Insulin-like growth factor I

Uniprot ID:

P05019

Data PDF:

CRI500

Source:

E. coli

Molecular Weight:

~7.6 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is < 2 ng/ml.

Specific Activity:

> 5 x 10e5 IU/mg

Endotoxin Level:

<1.0 EU/μg of recombinant human IGF1 as determined by LAL method.

Amino Acid Sequence:

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

The lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. After reconstitution, the preparation is stable for up to one week at 2-8°C or in aliquots at -20°C for longer term.