Growth hormone-dependent binding protein, IBP3, BP-53
Recombinant Human IFGBP3 is a single non-glycosylated polypeptide chain containing 264 amino acids.
Background: Insulin-like Growth Factor-Binding Protein 3(IGF-BP3) is a 30 kDa cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals and it is also found in α-granules of platelets. In addition to its ability to modulate the activity of IGF-I and IGF-II, IGF-BP3 exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF, and EGF.
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
>98% by SDS-PAGE and HPLC analyses.
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of ＞ 5.0 × 103 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
Less than 1EU/μg of rHuIGF-BP3 as determined by LAL method.
Amino Acid Sequence:
GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in appropriate buffered solutions.
Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots at -20°C-80°C. Avoid repeated freeze/thaw cycles.