Recombinant Human IL-1 alpha

Molecule:

IL-1 alpha

Synonyms:

Interleukin-1-alpha, IL-1A, IL1F1, IL1-alpha

Species:

Human

Cat. No.:

CRI132B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Recombinant Human IL1-alpha is a single non-glycosylated polypeptide chain containing 159 amino acids.

Background: Interleukin-1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1 alpha and IL1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL1 beta is a secreted cytokine, IL1 alpha is predominantly a cell-associated cytokine.

Data PDF:

CRI132

Source:

E. coli

Molecular Weight:

~18.0 kDa

Formulation:

Lyophilized from a sterile filtered solution in PBS, pH 7.5

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of > 1.0 × 109 IU/mg.

Endotoxin Level:

<1 EU/µg as determined by LAL method.

Amino Acid Sequence:

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.