Recombinant Human IL-1 beta

Molecule:

IL-1 beta

Synonyms:

Interleukin-1-beta, Catabolin, IL1-beta, IL1F2, IL-1 beta

Species:

Human

Cat. No.:

CRI133A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$135.00 BUY

Description:

Interleukin-1 beta (IL1β) is produced, mainly by activated macrophages, as a proprotein which is proteolytically processed to its active form by caspase 1. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar but not identical biological properties.

Recombinant Human IL-1 beta is a single non-glycosylated polypeptide chain containing 154 amino acids.

Entrez Symbol:

IL1B

Uniprot Name:

Interleukin-1 beta

Uniprot ID:

P01584

Data PDF:

CRI133

Source:

E. coli

Molecular Weight:

~17.5 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine D10S cells is less than 12 pg/ml.

Specific Activity:

> 8.3 x 107 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MAPVRSLNCT LRDSQQKSLV MSGPYELKAL HLQGQDMEQQ VVFSMSFVQG EESNDKIPVA LGLKEKNLYL SCVLKDDKPT LQLESVDPKN YPKKKMEKRF VFNKIEINNK LEFESAQFPN WYISTSQAEN MPVFLGGTKG GQDITDFTMQ FVSS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.