CSIF, TGIF, GVHDS, IL-10, IL10A
Interleukin-10 (IL-10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10 is a 178-aa molecule that contains two intra-chain disulfide bridges and is expressed as a 36 kDa non-covalently associated homodimer. The IL-10 dimer binds to two IL10-R-alpha/ IL-10R1 chains, resulting in recruitment of two IL-10 R-beta/ IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10R-beta does not bind IL-10 by itself but is required for signal transduction.
Recombinant Human IL-10 is a single non-glycosylated polypeptide chain containing 161 amino acids.
Lyophilized from a sterile-filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
>95% by SDS-PAGE and HPLC analyses.
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using mouse MC/9 cells is less than 5 ng/ml.
≥ 2 x 105 units/mg
Less than 1EU/μg as determined by kinetic LAL method.
Amino Acid Sequence:
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
The lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. After reconstitution, the preparation is stable for up to one week at 2-8°C or in aliquots at -20°C for longer term.