Recombinant Human IL-13 Variant

Molecule:

IL-13

Synonyms:

Interleukin-13, ALRH, BHR1, IL-13

Species:

Human

Cat. No.:

CSI20282A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$190.00 BUY

Description:

Recombinant Human Interleukin-13 Variant is a single, non-glycosylated polypeptide chain containing 112 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13.

Background: The human IL-13 cDNA encodes a 132 amino acid protein containing a proposed 20 amino acid signal peptide. Human IL-13 shares approximately 30% amino acid sequence homology to human IL-4 and the two cytokines exhibit overlapping biological activities. Human IL-13 is produced by activated Th0, Th1-like, Th2-like, and CD8 T cells. Similarly to IL-4, IL-13 has multiple effects on the differentiation and functions of monocytes/macrophages. IL-13 can suppress the cytotoxic functions of monocytes/macrophages. It can also suppress the production of proinflammatory cytokines and upregulate the production of IL-1ra by monocytes/macrophages.

Data PDF:

CSI20282

Source:

E. coli

Molecular Weight:

~12.3 kDa

Formulation:

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.2, containing 5% trehalose.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml.

Specific Activity:

>2.0 × 106 IU/mg

Endotoxin Level:

Less than 1EU/μg of rHuIL-13 variant as determined by LAL method.

Amino Acid Sequence:

GPVPPSTALR ELIEELVNIT QNQKAPLCNG SMVWSINLTA GMYCAALESL INVSGCSAIE KTQRMLSGFC PHKVSAGQFS SLHVRDTKIE VAQFVKDLLL HLKKLFREGQ FN

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C.