Recombinant Human IL-17

Molecule:

IL-17

Synonyms:

CTLA-8, IL17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8

Species:

Human

Cat. No.:

CRI162C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

This Product is Discontinued

Description:

Recombinant Human Interleukin-17A is a disulfide-linked homodimer of two 132 amino acid polypeptide chains.

Background: Human Interleukin 17A (IL-17A) is encoded by the IL17A gene located on chromosome 6 and belongs to the IL-17 family that comtains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. They have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, but no sequence similarity to any other known cytokines. Interleukin 17 is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology so a protein enclded by the ORF13 gene of herpesvirus Saimiri. Mature IL-17 contains one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, IL-17 exhibits 63% amino acid identity with mouse IL-17. High levels of human IL-17 were induced from primary peripheral blood CD4+ T cells upon stimulation and they can induce stromal cells to produce proinflammatory and hematopoietic cytokines.

Entrez Symbol:

IL17A

Uniprot Name:

Interleukin-17A

Uniprot ID:

Q16552

Data PDF:

CRI162

Source:

E. coli

Molecular Weight:

~31.0 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm sterile filtered solution in PBS, pH 7.4

Purity:

>95 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion by mouse NIH/3T3 cells is less than 7.5 ng/ml, corresponding to a specific activity of >1.3 x 105 units/mg.

Endotoxin Level:

<1 EU/μg as determined by LAL method.

Amino Acid Sequence:

GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C. Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.