Recombinant Human IL-1RA/IL-1RN

Molecule:

IL-1RA

Synonyms:

ICIL-1RA, IL1 inhibitor, IL1F3, IRAP, IL-1 RA

Species:

Human

Cat. No.:

CRI134C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$990.00 / Special Price: $395.00 BUY

Description:

Recombinant Human IL1RN is a single non-glycosylated polypeptide chain containing 152 amino acids.

Background: Interleukin-1 receptor antagonist (IL-1ra) is a member of the IL-1 family. Endogenous IL-1ra is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-ra. The regulated expression of IL-1ra in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1ra is markedly enhanced by IL-1, TNF-alpha, or PDGF.

Entrez Symbol:

IL1RN

Uniprot Name:

Interleukin-1 receptor antagonist protein

Uniprot ID:

P18510

Data PDF:

CRI134

Source:

E. coli

Molecular Weight:

~17.1 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution in PBS, pH 7.4

Purity:

>96% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 50 pg/ml rHuIL-1α.

Endotoxin Level:

Less than 1 EU/µg of rHuIL-1RA as determined by LAL method.

Amino Acid Sequence:

RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

The lyophilized protein is stable at 2-8°C. Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.