Aldesleukin, Lymphokine, IL-2, TCGF, T-cell Growth Factor
Interleukin-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. The receptor for IL-2 contains three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes.
Recombinant Human Interleukin-2 is a single non-glycosylated polypeptide chain containing 134 amino acids.
Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
>95% by SDS-PAGE and HPLC analysis.
As determined by the dose-dependant proliferation of mouse CTLL-2 cells is <3 ng/ml
≤1 U/μg of protein by kinetic LAL analysis (50% confidence range).
Amino Acid Sequence:
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT
Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C.