Recombinant Human IL-2

Molecule:

IL-2

Synonyms:

Aldesleukin, Lymphokine, IL-2, TCGF, T-cell Growth Factor

Species:

Human

Cat. No.:

CRI100C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$650.00 / Special Price: $395.00 BUY

Description:

Interleukin-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. The receptor for IL-2 contains three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes.
Recombinant Human Interleukin-2 is a single non-glycosylated polypeptide chain containing 134 amino acids.

Entrez Symbol:

IL2

Uniprot Name:

Interleukin-2

Uniprot ID:

P60568

Data PDF:

CRI100

Source:

E. coli

Molecular Weight:

~15.5 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

>95% by SDS-PAGE and HPLC analysis.

Biological Activity:

As determined by the dose-dependant proliferation of mouse CTLL-2 cells is <3 ng/ml

Endotoxin Level:

≤1 U/μg of protein by kinetic LAL analysis (50% confidence range).

Amino Acid Sequence:

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C.