Recombinant Human IL-21

Molecule:

IL-21

Synonyms:

Interleukin-21, Za11, IL-21

Species:

Human

Cat. No.:

CRI172A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$135.00 BUY

Description:

Recombinant Human IL-21 is a single, non-glycosylated polypeptide chain containing 132 amino acids.

Background: Interleukin 21 (IL-21) is a member of the common-gamma chain family of cytokines with immunoregulatory activity. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer (NK) cells, B cells and cytotoxic T cells. IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors. The IL-21R activates the JAK/STAT signaling pathway and is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 is a switch factor regulating IgG1 and IgG3 antibody production. IL-21 also cooperates with IL-4 for the production of multiple antibody classes in B cells.

Entrez Symbol:

IL21

Uniprot Name:

Interleukin-21

Uniprot ID:

Q9HBE4

Data PDF:

CRI172

Source:

E. coli

Molecular Weight:

~15.4 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered solution with 20 mM sodium phosphate, pH 7.5.

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by proliferation assay using ANBL-6 cells, is < 2 ng/ml.

Specific Activity:

>5.0 × 105 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.