Recombinant Human IL-22

Molecule:

IL-22

Synonyms:

IL-10-related T-cell-derived inducible factor, IL-22, IL-TIF, TIFa, ILTIF, IL-D110, TIFIL-23, zcyto18.

Species:

Human

Cat. No.:

CRI177B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Recombinant human IL-22 is a non-disulfide-linked homodimeric protein containing two 147 amino acid polypeptide chains.

Background: Interleukin-22 (IL-22) is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-β/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family.

Entrez Symbol:

IL22

Data PDF:

CRI177

Source:

E. coli

Molecular Weight:

~33.6 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.

Purity:

>97.0% by determined by SDS-PAGE and HPLC.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by IL-10 secretion by human Colo205 cells is less than 0.3 ng/ml, corresponding to a specific activity of >3.3 X 106 IU/mg.

Endotoxin Level:

Less than 1EU/μg of rHuIL-22 as determined by LAL method.

Amino Acid Sequence:

MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI

Reconstitution:

Centrifuge vial prior to opening to bring contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store lyophilized product desiccated at -20°C. Upon reconstitution, protein may be stored at 2-4°C for one week. Stock solutions should be apportioned into working aliquots and stored at -20°C to -80°C. Avoid repeated freeze-thaw cycles.