IL-10-related T-cell-derived inducible factor, IL-22, IL-TIF, TIFa, ILTIF, IL-D110, TIFIL-23, zcyto18.
Recombinant human IL-22 is a non-disulfide-linked homodimeric protein containing two 147 amino acid polypeptide chains.
Background: Interleukin-22 (IL-22) is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-β/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family.
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.
>97.0% by determined by SDS-PAGE and HPLC.
Fully biologically active when compared to standard. The ED50 as determined by IL-10 secretion by human Colo205 cells is less than 0.3 ng/ml, corresponding to a specific activity of >3.3 X 106 IU/mg.
Less than 1EU/μg of rHuIL-22 as determined by LAL method.
Amino Acid Sequence:
MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI
Centrifuge vial prior to opening to bring contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Store lyophilized product desiccated at -20°C. Upon reconstitution, protein may be stored at 2-4°C for one week. Stock solutions should be apportioned into working aliquots and stored at -20°C to -80°C. Avoid repeated freeze-thaw cycles.