Recombinant Human IL-33

Molecule:

IL-33

Synonyms:

interleukin-33, IL-33, IL-1F11, DVS27-related protein, interleukin-1 family member 11, nuclear factor from high endothelial venules

Species:

Human

Cat. No.:

CRI225C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$4,680.00 / Special Price: $2,590.00 BUY

Description:

Recombinant Human Interleukin-33 is a single non-glycosylated polypeptide chain containing 159 amino acids.

Background: IL-33, encoded by IL-33 gene located on the Chr.9 in humans, is a 30 kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20% a.a. sequence identity with other members. IL-33 secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 mature protein has 52-58% a.a. sequence identity with mouse and rat IL-33.

Data PDF:

CRI225

Source:

E. coli

Molecular Weight:

~17.9 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 150 mM NaCl, 1mM EDTA, pH7.4.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0 × 107 IU/mg.

Endotoxin Level:

<1 EU/μg of recombinant human IL-33 as determined by LAL method.

Amino Acid Sequence:

SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C. Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.