Recombinant Human IL-7

Molecule:

IL-7

Synonyms:

Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7

Species:

Human

Cat. No.:

CRI108C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,950.00 BUY

Description:

Recombinant IL-7 is a single polypeptide chain comprised of 153 amino acids.

Background: IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. IL-7 is constitutively produced by stromal cells from the bone marrow and thymus and can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells. IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2.

Entrez Symbol:

IL7

Uniprot Name:

Interleukin-7

Uniprot ID:

P13232

Data PDF:

CRI108

Source:

E. coli

Molecular Weight:

17.5 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution of 10mM acetic acid.

Purity:

>95% by SDS-PAGE

Biological Activity:

The ED50, as determined by the dose-dependent stimulation of the proliferation of mouse 2E8 cells, is less than 1 ng/ml.

Specific Activity:

>1 x 10e6 units/mg

Endotoxin Level:

< 1 EU/μg as determined by Kinetic LAL method.

Amino Acid Sequence:

MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. Allow several minutes for complete reconstitution. DO NOT VORTEX. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. After reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.