Replaced by CRI500 (Recombinant Human Insulin-like Growth Factor 1, Receptor Grade)

Molecule:

IGF-I

Synonyms:

IGF1 , IGF-I , IGFI , IGF-I , IGF1A

Species:

Human

Cat. No.:

CU100 pdf (datasheet)

Quantity/Size:

100 μg

Description:

Recombinant human IGF-1 is a single, non-glycosylated polypeptide containing 70 amino acids and 3 intra-molecular disulfide bonds.
Background: Insulin-like growth factor 1 (IGF-I) is a member of the IGF family of mitogenic peptide growth factors, along with insulin and IGF-II. IGF-I stimulates proliferation and survival of various cell types including muscle, bone, and cartilage tissue. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/ autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH). Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR). Proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions, generates mature IGFs.

Entrez Symbol:

IGF1

Data PDF:

CM001

Source:

E. coli

Molecular Weight:

~7.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic acid (TFA).

Purity:

>98% by SDS-PAGE and HPLC

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of mouse FDC-P1 cells, is typically less than 1.0 ng/ml.

Specific Activity:

> 1 x 10e6 U/mg

Endotoxin Level:

<1 EU/μg as determined by kinetic LAL analysis.

Amino Acid Sequence:

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized protein is stable at room temperature for shipping. On receipt, store desiccated at -20°C. After reconstitution, stable for one month at 2-8°C. For longer term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.