Recombinant Human Interferon Beta 1a

Molecule:

IFN-beta 1a

Synonyms:

Interferon beta 1a, Fibroblast interferon, IFNB

Species:

Human

Cat. No.:

CR2062 pdf (datasheet)

Quantity/Size:

1 x 10E6 U

Price:

$695.00 BUY

Description:

Recombinant human Interferon Beta 1a, mammalian (Hu IFN-β 1a)

Entrez Symbol:

IFNB1

Data PDF:

CR2062

Source:

CHO cells

Molecular Weight:

~23 kDa

Physical State:

Liquid

Formulation:

Liquid in 10 mM acetic acid.

Purity:

>95%, one N-linked glycosylation site

Biological Activity:

Biological Activity: 4.07 x 107 units/ml
Assay used to measure bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Familletti, et al. (1981) "A Convenience and Rapid Cytopathic Effect Inhibition Assay for Interferon, " in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. Encephalomyocarditis virus (EMCV) was used to challenge A549 cells [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. The activity was determined relative to a lab standard of Human IFN-beta which was calibrated to the NIH Human IFN-beta standard (Gb23-902-531). The EC50 for IFN is ~ 1 U/ml. Lot activity was derived from multiple determinations in the above assay. Please note that IFN assays vary between labs and assay systems [Meager et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1]

Specific Activity:

Lot Specific

Endotoxin Level:

< 1 EU/µg of protein

Storage/Stability:

Centrifuge vial prior to opening. After receipt, this product should be kept at 2-4°C for retention of full activity. Further dilution of this product for long term storage is not recommended.