Recombinant Human FGF7/KGF-1

Molecule:

FGF7

Synonyms:

Keratinocyte Growth Factor, Fibroblast Growth Factor-7, FGF7, FGF-7, HBGF-7, SDGF-3

Species:

Human

Cat. No.:

CRK300A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$135.00 BUY

Description:

Recombinant Human KGF-1 is a single, non-glycosylated polypeptide chain containing 164 amino acids.

Background: Human KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. KGF/FGF-7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.

Entrez Symbol:

FGF7

Uniprot Name:

Keratinocyte growth factor

Uniprot ID:

P21781

Data PDF:

CRK300

Source:

E. coli

Molecular Weight:

~18.9 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered aqueous solution containing 10 mM sodium phosphate + 150 mM sodium chloride, pH 7.5.

Purity:

> 90% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of monkey 4MBr-5 cells is typically 2-3 ng/ml.

Specific Activity:

> 5 x 105 U/mg.

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.