Recombinant Human LONG®R3 IGF-I (Liquid)

Molecule:

IGF1

Synonyms:

IGF-IA, IGFI, IGF1

Species:

Human

Cat. No.:

CRH061 pdf (datasheet)

Quantity/Size:

5 ml

Price:

$785.00 BUY

Description:

Recombinant Human LONG®R3 IGF-I (Liquid) is a cell culture supplement for use in serum-free media to enhance survival and proliferation of mammalian cells.

Background: LONG®R3 IGF-I is an analog of IGF-1 that was developed for use in mammalian serum-free cell culture to promote cell growth, survival, and recombinant protein expression. When supplemented in serum-free medium, it promotes cell proliferation, increases cell survival, and enhances productivity through better proliferative and anti-apoptotic signaling. It has an increased half-life in vitro and in vivo and a low affinity for IGF binding proteins (IGFBP) that make it ideal for both research and large-scale culturing.

The protein was created by substituting an arginine (R) for glutamic acid (E) at amino acid 3 and extending the N-terminal end of the peptide by 13 amino acids (MFPAMPLSSLFVN). All cell types that have a growth response to insulin in cell culture have the potential to respond to LONG®R3 IGF-I. Recombinant Human LONG®R3 IGF-I is a single, non-glycosylated polypeptide chain containing 83 amino acids.

Concentration:

0.9 - 1.1 mg/ml

Entrez Symbol:

IGF1

Data PDF:

CRH061

Source:

E. coli

Molecular Weight:

~9.1 kDa as determined by mass spectrometry (9108-9112 Da)

Formulation:

Clear liquid in a glass vial, type 1.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis in L6 myoblasts is <10 ng/ml.

Endotoxin Level:

<0.1 EU/μg as determined by LAL assay

Amino Acid Sequence:

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Storage/Stability:

Store at 2 - 8 °C. Stable for 4 years.

Application notes:

Ready to use. Simply open and add directly to cell culture medium.