Recombinant Human LR3 IGF-1

Molecule:

IGF1

Synonyms:

Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF

Species:

Human

Cat. No.:

LRM050 pdf (datasheet)

Quantity/Size:

50 mg

Price:

$3,000.00 BUY

Description:

Recombinant Human LR3 IGF-1 is a single, non-glycosylated polypeptide chain containing 83 amino acids. Background: IGF-1 belongs to the insulin gene family and is a mitogenic polypeptide growth factor that stimulates the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine manner. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3 IGF-1 is a long-term acting analog of human IGF-1 that is specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.

Data PDF:

LRM001

Source:

E. coli

Molecular Weight:

~9.1 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2.

Purity:

>98 % by SDS-PAGE analyses. >90 % by RP-HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0

Amino Acid Sequence:

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilut

Storage/Stability:

This lyophilized preparation is stable for 5 years at 2-8 °C. Upon reconstitution, the preparation is stable for up to one month at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20