Recombinant Human M-CSF/CSF1

Molecule:

CSF1

Synonyms:

MCSF, CSF-1

Species:

Human

Cat. No.:

CRM151B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Macrophage Colony Stimulating Factor (M-CSF), also called CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone), exerts paracrine effects on osteoclasts, and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer thought to be produced by proteolytic cleavage of membrane-bound precursors.

Recombinant human M-CSF is a disulfide-linked homodimer consisting of two 159 amino acid polypeptide chains.

Entrez Symbol:

CSF1

Uniprot Name:

Macrophage colony-stimulating factor 1

Uniprot ID:

P09603

Data PDF:

CRM151

Source:

E. coli

Molecular Weight:

37.1 kDa (dimer) / 18.5 kDa (monomer)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution 10 mM sodium phosphate + 100 mM sodium chloride, pH 8.0

Purity:

>95 % by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a a cell proliferation assay using mouse NFS-60 cells is less than 10 ng/ml.

Specific Activity:

> 1.0 × 105 IU/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.