Recombinant Human MCP4/CCL13

Molecule:

CCL13

Synonyms:

Monocyte Chemoattractant Protein 4, Monocyte Chemotactic Protein 4, CKb10, CK-beta-10, NCC1, SCYA13, SCYL1

Species:

Human

Cat. No.:

CRM005B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$235.00 BUY

Description:

Recombinant Human MCP4/CCL13 is a single non-glycosylated polypeptide chain containing 75 amino acids.

Background: MCP-4/CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4+ and CD8+ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.

Data PDF:

CRM005

Source:

E. coli

Molecular Weight:

~8.6 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.

Purity:

>96 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuMCP-4/CCL13 as determined by LAL method.

Amino Acid Sequence:

QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.