Recombinant Human NAP-2/CXCL7

Molecule:

CXCL7

Synonyms:

Neutrophil-Activating Peptide 2, Pro-Platelet Basic Protein, PPBP, CXCL7, B-TG1, CTAP3, SCYB7

Species:

Human

Cat. No.:

CRN600A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$190.00 BUY

Description:

Recombinant Human NAP-2/CXCL7 is a single non-glycosylated polypeptide chain containing 70 amino acids.

Background: Neutrophil Activating Peptide 2 (NAP-2) is proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR-2 and to chemoattract and activate neutrophils. Although CTAP-III, beta-TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. Recently, it has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

Data PDF:

CRN600

Source:

E. coli

Molecular Weight:

~7.6 kDa

Formulation:

Lyophilized from a sterile filtered solution in 20 mM PB, pH 7.4 + 50 mM NaCl

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 1.0-10.0 ng/ml.

Endotoxin Level:

Less than 1 EU/μg of rHuNAP-2/CXCL7 as determined by LAL method.

Amino Acid Sequence:

AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-4°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-4°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.