Recombinant Human Neurotrophin 4

Molecule:

NTF4

Synonyms:

NT-4/5, NT4, NT5, NTF5, neurotrophic factor 4, neurotrophic factor 5, neurotrophin 5, neurotrophin 5 (neurotrophin 4/5)

Species:

Human

Cat. No.:

CRN501C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,950.00 BUY

Description:

Recombinant Human Neurotrophin-4 is a non-covalently linked homodimer composed of two 14.0 kDa polypeptide monomers for a total of 262 amino acids.

Background: Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. Human NT-4 shares 48 - 52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 promotes the development and survival of selected peripheral and CNS neurons. NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death. It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle. NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration.

Data PDF:

CRN501

Source:

E. coli

Molecular Weight:

~28.1 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.5.

Purity:

>97 % by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml.

Endotoxin Level:

Less than 1 EU/μg as determined by LAL method.

Amino Acid Sequence:

MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.

Storage/Stability:

This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C.