Recombinant Human PDGF-BB

Molecule:

PDGFB

Synonyms:

PDGF-2, PDGF2, SIS, SSV, IBGC5, c-sis, GDGF, ODGF

Species:

Human

Cat. No.:

CRP302B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Recombinant Human PDGF-BB is a non-glycosylated, disulfide-linked homodimer containing two 110 amino acid chains.
Background: Platelet-Derived Growth Factor (PDGF) is a member of the protein family which includes vascular endothelial growth factors (VEGF). PDGF is proteolytically processed from a pre/pro-protein to generate PDGF subunit B, which can homodimerize, or can heterodimerize with the related PDGF subunit A. These proteins bind and activate PDGF receptor tyrosine kinases. PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets, released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF.

Entrez Symbol:

PDGFB

Uniprot Name:

Platelet-derived growth factor subunit B

Uniprot ID:

P01127

Data PDF:

CRP302

Source:

E. coli

Molecular Weight:

24.3 kDa (two 12.5 kDa chains)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution with 10 mM sodium phosphate, pH 7.5

Purity:

>95.0% as determined by reducing and non-reducing SDS-PAGE

Biological Activity:

The activity is determined by the dose-dependent proliferation of 3T3 indicator cells and is typically 1.5-2.2 ng/ml.

Endotoxin Level:

Measured by kinetic LAL analysis and is typically ≤1 EU/μg protein.

Amino Acid Sequence:

MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT

Reconstitution:

Centrifuge vial prior to opening. Reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Storage/Stability:

Lyophilized protein is stable at room temperature for shipping. On receipt, store desiccated at -20°C. After reconstitution, stable for one month at 2-8°C. For longer term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.