Recombinant Human Protein Tyrosine Phosphatase 1B (aa 1-321)

Molecule:

PTP-1B

Synonyms:

PTP1B

Species:

Human

Cat. No.:

CSI15622A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$350.00 BUY

Description:

Recombinant Human Protein Tyrosine Phosphatase, non-receptor type 1 (aa 1-321) The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.

Concentration:

1 mg/ml (determined by Bradford assay)

Entrez Symbol:

PTPN1

Data PDF:

CSI15622

Genbank Accession No:

NP_002818

Source:

E. coli

Molecular Weight:

37.3 kDa (321 aa)

Physical State:

Liquid

Formulation:

Liquid formulation in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM beta-mercaptoethanol, 1 mM EDTA, 20%Glycerol

Purity:

> 95% by SDS - PAGE

Biological Activity:

Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37οΨΑC, pH7.5 using 10mM of substrate.

Specific Activity:

6,000-12,000 units/mg

Amino Acid Sequence:

MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CL

Reconstitution:

NA

Storage/Stability:

Can be stored at +4οΨΑC short term (1-2 weeks). For long term storage, aliquot and store at -20οΨΑC or -80οΨΑC. Avoid repeated freezing and thawing cycles.