Recombinant Human RANTES/CCL5

Molecule:

CCL5

Synonyms:

RANTES, SCYA5, SISd, TCP228, SIS-delta, T-cell specific protein p288, Regulated upon activation, normally T-expressed, and presumably secreted, Small inducible cytokine A5

Species:

Human

Cat. No.:

CRR000C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 / Special Price: $1,640.00 BUY

Description:

Recombinant Human RANTES/CCL5 is a single non-glycosylated polypeptide chain containing 68 amino acids.

Background: RANTES/CCL5 (acronym for Regulated upon Activation, Normal T cell Expressed and presumably Secreted), was initially discovered by subtractive hybridization as a transcript expressed in T cells but not B cells. Eosinophil chemotactic activities released by thrombin stimulated human platelets have also been purified and found to be identical to RANTES. Besides T cells and platelets, RANTES has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells.

Data PDF:

CRR000

Source:

E. coli

Molecular Weight:

~7.8 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4 + 100 mM NaCl.

Purity:

>98% as determined by HPLC and SDS-PAGE analyses

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.

Endotoxin Level:

Less than 1EU/μg of recombinant human RANTES/CCL5 as determined by LAL method.

Amino Acid Sequence:

SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.