Recombinant Human RAP Stable Mutant

Molecule:

RAP

Synonyms:

RAP, A2MRAP, A2RAP, MRAP, HBP44

Species:

Human

Cat. No.:

CRH062B pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,760.00 BUY

Description:

Human Receptor Associated Protein (RAP) is a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. The stable mutant form of human RAP is resistant to both pH and heat induced denaturation. The molecule binds to lipoprotein receptor-related protein 1 (LRP1) with high affinity and inhibits LRP1 function both in vitro and in vivo. The D3 domain of RAP, which unfolds at low pH causing dissociation from LRP1, has been stabilized with six mutations. Y260 and T297 are changed to cysteines to generate a novel disulfide bond between helices 2 and 3 whereas H257, H259, H268, and H290 are changed to phenylalanines. Human RAP Stable Mutant does not unfold at pH 5.5 and has a melting temperature of 73°C, more than 30 degrees above that of wild type RAP. It is also more resistant to trypsin and chymotrypsin mediated proteolysis.

Concentration:

2.5 mg/ml

Entrez Symbol:

LRPAP1

Data PDF:

CRH062

Source:

E. coli

Molecular Weight:

39 kDa

Formulation:

Frozen Liquid in 0.1 M Tris-HCl + 0.15 M NaCl, pH7.4

Purity:

>90% by SDS-PAGE analysis

Endotoxin Level:

Contains endotoxin which might trigger signaling events.

Storage/Stability:

Store at -70°C. Stable for 3 years from delivery. Avoid repeated freeze-thaw cycles.

Application notes:

Recombinant Human RAP Stable Mutant may be useful in multiple pathological settings where LRP1 blockade has shown to be effective.