Recombinant Human KIT Ligand/SCF

Molecule:

SCF

Synonyms:

KIT Ligand, KITLG, SF, Clo, Con, Gb, Kitl, Kl2, MGF, SLF, SCF

Species:

Human

Cat. No.:

CRS200A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$135.00 BUY

Description:

C-kit ligand, also known as stem cell factor (SCF), is the ligand of the KIT tyrosine-kinase receptor. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. In vitro and in vivo, SCF can stimulate the proliferation of mature, as well as the proliferation and maturation of immature, mast cells. On purified human and mouse hematopoietic precursors, SCF acts in a synergistic manner with various growth factors, such as IL-1, IL-3, IL-6, IL-7, and Epo, to induce myeloid, erythroid and lymphoid lineage colony formation. SCF is also expressed in the nervous system suggests a possible role for SCF in the development of the nervous system.

Recombinant human KIT-ligand is a single polypeptide chain containing 165 amino acids.

Entrez Symbol:

KITLG

Uniprot Name:

Kit ligand

Uniprot ID:

P21583

Data PDF:

CRS200

Source:

E. coli

Molecular Weight:

~18.6 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 10 mM acetic acid.

Purity:

>95% by SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using human TF-1 cells is less than 15 ng/ml.

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis.

Amino Acid Sequence:

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.