Recombinant Human Sclerostin His

Molecule:

SOST

Synonyms:

CDD1οΨ†, VBCH

Species:

Human

Cat. No.:

CRH013C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$6,180.00 BUY

Description:

Sclerostin (SOST) is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin functions as a negative regulator of bone growth, by inhibiting bone formation. SOST is widely expressed at low levels, with highest levels in the bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. SOST gene defects cause sclerosteosis and bone dysplasia. Recombinant Human Sclerostin is a single, non-glycosylated, polypeptide chain consisting of aa24-213 (200 aa) including a 10 aa N-terminal His tag.

Entrez Symbol:

SOST

Data PDF:

CRH013

Source:

E. coli

Molecular Weight:

22.8 kDa (calculated)

Tag:

His-tagged

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.4 οΨΒm filtered concentrated (0.5 mg/ml) solution containing 0.03 M acetate buffer, pH 4.0.

Purity:

>90% by densitometric image analysis.

Amino Acid Sequence:

MKHHHHHHASQGWQAFKNDA TEIIPELGEY PEPPPELENN KTMNRAENGG RPPHHPFETK DVSEYSCREL HFTRYVTDGP CRSAKPVTEL VCSGQCGPAR LLPNAIGRGK WWRPSGPDFR CIPDRYRAQR VQLLCPGGEA PRARKVRLVA SCKCKRLTRF HNQSELKDFG TEAARPQKGR KPRPRARSAK ANQAELENAY

Reconstitution:

Centrifuge vial prior to opening. It is recommended to add 0.1 M acetate buffer, pH 4, to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend int

Storage/Stability:

Store lyophilized protein at -20οΨΑC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored for 2 weeks at 4οΨΑC. Avoid repeated freeze/thaw cycles.