Recombinant Human SMARCA4 His

Molecule:

SMARCA4

Synonyms:

BAF190 , BAF190A , BRG1 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b

Species:

Human

Cat. No.:

CSI13911 pdf (datasheet)

Quantity/Size:

5 μg

Price:

$910.00 BUY

Description:

The wild type human brahma-related gene 1 encodes a protein of 1,647 amino acids that contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription regulation. BRG1 is an essential component of the SWI/SNF chromatin remodeling complex and is implicated in multiple functions through its interaction with different proteins, including the tumor suppressor protein pRb, serine-threonine kinase LKB1, and other transcription factors. Although Brg1 is involved in chromatin remodeling complexed with other SWI/SNF proteins, purified BRG1 itself is capable of remodeling mononucleosomes and nucleosomal arrays in vitro. Mutations of Brg1 have been found in multiple tumor cell lines.

Entrez Symbol:

SMARCA4

Data PDF:

CSI13911

Source:

Sf9 insect cells

Tag:

His-tagged

Physical State:

Liquid

Formulation:

Frozen liquid in 20 mM Tris-HCl, pH 7.9 + 100 mM KCl + 0.2 mM EDTA + 1 mM DTT + 20% glycerol

Purity:

> 90% as determined by SDS-PAGE analysis.

Storage/Stability:

Stable for 1 year in working aliquots at -80οΨΑC. Avoid repeated freeze-thaw cycles.

Application notes:

Protein-protein interaction assay In vitro transcription assay In vitro nucleosome remodeling assay Cell growth assay Protein-protein interaction assay requires 100 ng. In vitro nucleosome remodeling assay requires 50-100 ng.