Recombinant Human SMARCA4 K798R Mutant His

Molecule:

SMARCA4

Synonyms:

BAF190 , BAF190A , BRG1 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b

Species:

Human

Cat. No.:

CSI13912 pdf (datasheet)

Quantity/Size:

5 μg

Price:

$840.00 BUY

Description:

BRG1 is an essential component of the SWI/SNF chromatin remodeling complex and implicated in multiple functions through its interaction with different proteins, including the tumor suppressor protein pRb, serine-threonine kinase LKB1, and other transcription factors. Mutation of lysine to arginine at position 798 in the DNA-dependent ATPase domain of BRG1 a) failed to restore normal growth to swi2-cells, b) reduced its ability to repress c-fos transcription and c) reduced CIITA (class II transactivator) induction and enhanced the rate of induction of the interferon-gamma-responsive GBP-1 gene.

Entrez Symbol:

SMARCA4

Data PDF:

CSI13912

Source:

Sf9 insect cells

Tag:

His-tagged

Physical State:

Liquid

Formulation:

Frozen liquid in 20 mM Tris-HCl, pH 7.9 + 100 mM KCl + 0.2 mM EDTA + 1 mM DTT + 20% glycerol

Purity:

> 90% as determined by SDS-PAGE analysis.

Storage/Stability:

Stable for 1 year in working aliquots at -80οΨΑC. Avoid repeated freeze-thaw cycles.

Application notes:

Protein-protein interaction assay In vitro transcription assay In vitro nucleosome remodeling assay Cell growth assay 100 ng are required for a protein-protein interaction assay.